Composite

Part:BBa_I716225:Design

Designed by: Samantha Liang   Group: iGEM07_Berkeley_UC   (2007-06-26)


[rbs library][Cre]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

GTG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)

Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)

Plate on CamAmp

Product is pBca9145-I716225.



Source

GTG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)

Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)

Plate on CamAmp

Product is pBca9145-I716225.


References